At Home Tissue Culture

At Home Tissue Culture

At Home Tissue Culture

At Home Tissue Culture
In this episode of the Canna Cribs Podcast, Nick talks with David Gonzalez and we get to learn how to make your own Agar recipe at home, tissue culture and much more! Enjoy!

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What’s in the interview?

Hello David, please tell us a little bit about yourself.
A botanist degree from Ohio University, what was your discipline?
Do you feel everyone needs a degree in botany to understand TC?
What kind of barrier to entry is someone looking at?

You are an expert in tissue culture – what is tissue culture?
Can you outline the basic process?
What is the Callus method?
What does a TC SOP look like?
What is the process of making an agar solution at home?
Can you give us an example of an agar recipe for our listeners/viewers?
Do you just take a clone and sit it out in the water and wait till it roots or gets a node?
I’d like to talk a little more about the disease resistance of tissue culture –
I read that tissue cultures are taken from meristematic tissue, which helps prevent viral diseases. What makes the tissue different from others?
How does it work to create disease resistance?

What kind of benefits has been shown outside the cannabis space?
More and more cannabis growers are turning to tissue culture, what are the advantages of TC in-home use, small business, and large-scale production?
How do you utilize TC at scale?
What is genetic drift?

I’ve heard you are passionate about and have a vast knowledge of genetic engineering specifically for cannabis, how does tissue culture play a role in this?
Can you tell me about your plan to make a powdery mildew-resistant strain?
How do you engineer your terpenes?
What is Golden Rice?

What are your predictions for the future of tissue culture in the cannabis space?
# of growers adopting the technology?
The fate of seed companies?
What is QPCR?

Is there anything else you’d like to talk about that we didn’t get a chance to discuss?
Do you have any new projects or plans you can tell us about?
Any new tech coming out that will impact the tissue culture process?


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